White paper

Modeling and Online Estimation of kLa and P/V for 250mL and 5L Mammalian Bioreactors

In this study, we quantify the volumetric mass transfer coefficient of oxygen (ka) and specific power input (P/V) in bioreactors. These are essential parameters that define a bioreactor's operating conditions and are used to address bioprocess development challenges, namely, developing scale-up/scale-down models and tech-transfer. These parameters are difficult to measure in real-time.Using two datasets from Culture Biosciences' 250-mL and 5-L mammalian bioreactors, we modeled kLa and P/V based on three variables: agitation, air sparging flow rate, and reactor volume. Three modeling techniques were explored: polynomial regression, a power and gassing kLa correlation, andGaussian Kernel Regression. The non-parametric Gaussian Kernel Regression demonstrated flexibility in capturing complex, non-linear relationships. We utilize Gaussian Kernel Regression models to compare kLa, P/V, and oxygen transfer rates between 250-mL and 5-L bioreactor scales fora Chinese Hamster Ovary cell (CHO) process. These kLa and P/V models allow existing clients to gain insight to scale-up related parameters, and assist new clients in transferring their processes ontoCulture’s platform.

Complete the form to download the full PDF

Keep exploring

White paper

AAV Production in Culture Biosciences’ 250mL Bioreactors

January 17, 2024
White paper

A Reproducible and Scalable System for CHO Cell Culture