Human embryonic kidney cells are widely used in gene therapy and other therapeutic expression processes. They possess several key attributes that make them good hosts for genetic modification and gene therapy expression. Here we describe the growth of suspension adapted HEK293 cells in 250mL and 5L bioreactors. We monitored viable cell density and viability along with metabolites ammonium, lactate, and glutamine. All measurements reached the same end points, with viable cell density, viability, lactate and glutamine following similar trends between scales.