--- title: "Unveiling True Growth and Production Rates: Precision Volume Accounting In Fed-Batch Cultures" description: Estimation of process rates is a key part of data analysis for bioprocess experiments. image: https://www.culturebiosciences.com/hubfs/Imported_Blog_Media/659b514b5f7a4947e7012e1c_Image-2-1.png --- [Skip to main content](https://www.culturebiosciences.com/resources/unveiling-true-growth-and-production-rates-precision-volume-accounting-in-fed-batch-cultures#main-content) [![Culture Biosciences](https://www.culturebiosciences.com/hubfs/2026_Culture%20Biosciences/Logos/Culture%20Logo%20black.svg) ![Culture Biosciences](https://www.culturebiosciences.com/hubfs/2026_Culture%20Biosciences/Logos/Culture%20Logo%20white.svg) ![Culture Biosciences](https://www.culturebiosciences.com/hubfs/2026_Culture%20Biosciences/Logos/Culture%20Logo%20black.svg)](https://www.culturebiosciences.com) - Show submenu for Products Products - [Console Software](https://www.culturebiosciences.com/console) - [Stratyx Bioreactor](https://www.culturebiosciences.com/stratyx) - [Services](https://www.culturebiosciences.com/nexxys) - Show submenu for Resources Resources - [White Papers](https://www.culturebiosciences.com/resources/tag/white-paper) - [Technical Bulletins](https://www.culturebiosciences.com/resources/tag/technical-bulletin) - [Case Studies](https://www.culturebiosciences.com/resources/tag/case-study) - [Blog Posts](https://www.culturebiosciences.com/resources/tag/blog-post) - [Posters](https://www.culturebiosciences.com/resources/tag/poster) - [Announcements](https://www.culturebiosciences.com/resources/tag/announcement) - [Webinars](https://www.culturebiosciences.com/resources/tag/webinar) - [Videos](https://www.culturebiosciences.com/resources/tag/video) - [View All](https://www.culturebiosciences.com/resources) - [About](https://www.culturebiosciences.com/about) Open main navigation Close main navigation - Show submenu for Products Products - Products - [Console Software](https://www.culturebiosciences.com/console) - [Stratyx Bioreactor](https://www.culturebiosciences.com/stratyx) - [Services](https://www.culturebiosciences.com/nexxys) - Show submenu for Resources Resources - Resources - [White Papers](https://www.culturebiosciences.com/resources/tag/white-paper) - [Technical Bulletins](https://www.culturebiosciences.com/resources/tag/technical-bulletin) - [Case Studies](https://www.culturebiosciences.com/resources/tag/case-study) - [Blog Posts](https://www.culturebiosciences.com/resources/tag/blog-post) - [Posters](https://www.culturebiosciences.com/resources/tag/poster) - [Announcements](https://www.culturebiosciences.com/resources/tag/announcement) - [Webinars](https://www.culturebiosciences.com/resources/tag/webinar) - [Videos](https://www.culturebiosciences.com/resources/tag/video) - [View All](https://www.culturebiosciences.com/resources) - [About](https://www.culturebiosciences.com/about) - [Login](https://console.culturebiosciences.com/login) [Contact](https://www.culturebiosciences.com/contact) [Login](https://console.culturebiosciences.com/login) [Contact](https://www.culturebiosciences.com/contact) White Paper December 3, 2024 # Unveiling True Growth and Production Rates: Precision Volume Accounting In Fed-Batch Cultures ![Unveiling True Growth and Production Rates: Precision Volume Accounting In Fed-Batch Cultures](https://www.culturebiosciences.com/hs-fs/hubfs/Imported_Blog_Media/659b514b5f7a4947e7012e1c_Image-2-1.png?width=860&height=745&name=659b514b5f7a4947e7012e1c_Image-2-1.png) Estimation of process rates (e.g. growth rate, glucose consumption rate, target molecule production rate) is a key part of data analysis for bioprocess experiments. Common approaches for rate calculation assume batch systems with no liquid addition or sampling drawdowns. These approaches, when not used carefully, can suffer from estimation errors when addition or sampling does in fact take place in a process, especially in small-scale bioreactors. This white paper describes a simple method to correct for volume changes in fed-batch systems, allowing for accurate estimation of process rates. Culture Biosciences carefully keeps track of volume changes throughout bioreactor experiments and provides all the data needed to perform these calculations correctly. ## Complete the form to download the full PDF ## Keep exploring [See all](https://www.culturebiosciences.com/resources) [![High-Density TFF Perfusion on the Stratyx 250 Platform](https://www.culturebiosciences.com/hs-fs/hubfs/Perfusion-Featured-Image-1.png?width=500&height=500&name=Perfusion-Featured-Image-1.png) White Paper High-Density TFF Perfusion on the Stratyx 250 Platform July 20, 2026](https://www.culturebiosciences.com/resources/high-density-tff-perfusion-on-the-stratyx-250-platform) [![Real-Time Closed-Loop Optimization Delivers 43% Titer Improvement](https://www.culturebiosciences.com/hs-fs/hubfs/Ark-TB-Thumbnail.png?width=500&height=500&name=Ark-TB-Thumbnail.png) White Paper Real-Time Closed-Loop Optimization Delivers 43% Titer Improvement May 5, 2026](https://www.culturebiosciences.com/resources/real-time-closed-loop-optimization-delivers-43-titer-improvement) [![STRATYX™ Delivers Superior Viable Cell Density and Increased mAb Titer Compared with Conventional 250 mL Bioreactors in Fed-Batch CHO Culture.](https://www.culturebiosciences.com/hs-fs/hubfs/Bionova-Thumbnail.png?width=500&height=500&name=Bionova-Thumbnail.png) White Paper STRATYX™ Delivers Superior Viable Cell Density and Increased mAb Titer Compared with Conventional 250 mL Bioreactors in Fed-Batch CHO Culture. April 15, 2026](https://www.culturebiosciences.com/resources/250ml-bioreactor-comparison) [![Stratyx™ 250 Inline Viable Cell Density Measurement](https://www.culturebiosciences.com/hs-fs/hubfs/VCD-WP-Thumbnail.png?width=500&height=500&name=VCD-WP-Thumbnail.png) White Paper Stratyx™ 250 Inline Viable Cell Density Measurement March 11, 2026](https://www.culturebiosciences.com/resources/stratyx-250-inline-viable-cell-density-measurement) [![Growth and Site to Site Comparison of a HEK Fed-Batch Process in Stratyx™ 250](https://www.culturebiosciences.com/hs-fs/hubfs/Imported_Blog_Media/6883edf69ed1f80d9fa6e613_Growth%20and%20Site%20to%20Site%20Comparison%20of%20a%20HEK%20Fed-Batch%20Process%20in%20Stratyx%E2%84%A2%20250.png?width=500&height=500&name=6883edf69ed1f80d9fa6e613_Growth%20and%20Site%20to%20Site%20Comparison%20of%20a%20HEK%20Fed-Batch%20Process%20in%20Stratyx%E2%84%A2%20250.png) White Paper Growth and Site to Site Comparison of a HEK Fed-Batch Process in Stratyx™ 250 July 25, 2025](https://www.culturebiosciences.com/resources/growth-and-site-to-site-comparison-of-a-hek-fed-batch-process-in-stratyx-tm-250) ![Culture Logo black](https://www.culturebiosciences.com/hubfs/2026_Culture%20Biosciences/Logos/Culture%20Logo%20black.svg) ### Sign up for our newsletter and grow your insights with Culture. Smarter bioprocessing starts here. - Services Packages - [Clone, strain and construct screening](https://www.culturebiosciences.com/services/clone-strain-and-construct-screening) - [Process characterization](https://www.culturebiosciences.com/services/process-characterization) - [Process development](https://www.culturebiosciences.com/services/process-development) - [Bioprocess modeling
and simulation](https://www.culturebiosciences.com/services/process-model-development) - Products - [StratyxTM Bioreactor](https://www.culturebiosciences.com/stratyx) - [Console Software](https://www.culturebiosciences.com/#console) - [NexxysTM](https://www.culturebiosciences.com/nexxys) - [250mL bench-scale single-use bioreactor](https://www.culturebiosciences.com/bioreactors/250ml) - [5L single-use bioreactor](https://www.culturebiosciences.com/bioreactors/5l) - Applications - [Synthetic biology applications](https://www.culturebiosciences.com/applications/synthetic-biology) - [Protein therapeutics](https://www.culturebiosciences.com/applications/protein-therapeutics) - [Viral vectors](https://www.culturebiosciences.com/applications/viral-vectors) - Resources - [White papers](https://www.culturebiosciences.com/resources/tag/white-paper) - [Case studies](https://www.culturebiosciences.com/resources/tag/case-study) - [Blog posts](https://www.culturebiosciences.com/resources/tag/blog-post) - [Announcements](https://www.culturebiosciences.com/resources/tag/announcement) - [Webinars](https://www.culturebiosciences.com/resources/tag/webinar) - [Videos](https://www.culturebiosciences.com/resources/tag/video) - [All resources](https://www.culturebiosciences.com/resources) - Company - [About](https://www.culturebiosciences.com/about) - [Careers](https://www.culturebiosciences.com/careers) - [Contact](https://www.culturebiosciences.com/contact) - [LinkedIn](https://www.linkedin.com/company/18240703/admin/feed/posts/) All systems operational... © 2026 Culture Biosciences [Terms](https://www.culturebiosciences.com/terms-of-service) [Privacy](https://www.culturebiosciences.com/privacy-policy) ```json { "@context" : "https://schema.org", "@type" : "BlogPosting", "author" : { "@type" : "Person", "name" : "Admin", "url" : "https://www.culturebiosciences.com/resources/author/admin" }, "dateModified" : "2026-02-24T06:14:53.117Z", "datePublished" : "2024-12-04T05:00:00.000Z", "headline" : "Unveiling True Growth and Production Rates: Precision Volume Accounting In Fed-Batch Cultures", "image" : [ "https://www.culturebiosciences.com/hubfs/Imported_Blog_Media/659b514b5f7a4947e7012e1c_Image-2-1.png" ], "mainEntityOfPage" : { "@id" : "https://www.culturebiosciences.com/resources/unveiling-true-growth-and-production-rates-precision-volume-accounting-in-fed-batch-cultures", "@type" : "WebPage" }, "publisher" : { "@type" : "Organization", "logo" : { "@type" : "ImageObject", "url" : "https://www.culturebiosciences.com/hubfs/Culture%20Biosciences%20Logo%202.png" } } } ```