--- title: Continuous in-situ pCO₂ monitoring on the Stratyx™ 250 platform description: Together, DOE-based exploration of the design space and seamless scale up ensure robustness and speed of process development. image: https://www.culturebiosciences.com/hubfs/White%20Paper%20Feature%20%E2%80%93%20Gradient%20Background%20(15).png --- [Skip to main content](https://www.culturebiosciences.com/resources/continuous-in-situ-pco-monitoring-on-the-stratyx-250-platform#main-content) [![Culture Biosciences](https://www.culturebiosciences.com/hubfs/2026_Culture%20Biosciences/Logos/Culture%20Logo%20black.svg) ![Culture Biosciences](https://www.culturebiosciences.com/hubfs/2026_Culture%20Biosciences/Logos/Culture%20Logo%20white.svg) ![Culture Biosciences](https://www.culturebiosciences.com/hubfs/2026_Culture%20Biosciences/Logos/Culture%20Logo%20black.svg)](https://www.culturebiosciences.com) - Show submenu for Products Products - [Console Software](https://www.culturebiosciences.com/-console) - [Stratyx Bioreactor](https://www.culturebiosciences.com/stratyx) - [Services](https://www.culturebiosciences.com/nexxys) - Show submenu for Resources Resources - [White Papers](https://www.culturebiosciences.com/resources/tag/white-paper) - [Technical Bulletins](https://www.culturebiosciences.com/resources/tag/technical-bulletin) - [Case Studies](https://www.culturebiosciences.com/resources/tag/case-study) - [Blog Posts](https://www.culturebiosciences.com/resources/tag/blog-post) - [Posters](https://www.culturebiosciences.com/resources/tag/poster) - [Announcements](https://www.culturebiosciences.com/resources/tag/announcement) - [Webinars](https://www.culturebiosciences.com/resources/tag/webinar) - [Videos](https://www.culturebiosciences.com/resources/tag/video) - [View All](https://www.culturebiosciences.com/resources) - [About](https://www.culturebiosciences.com/about) Open main navigation Close main navigation - Show submenu for Products Products - Products - [Console Software](https://www.culturebiosciences.com/-console) - [Stratyx Bioreactor](https://www.culturebiosciences.com/stratyx) - [Services](https://www.culturebiosciences.com/nexxys) - Show submenu for Resources Resources - Resources - [White Papers](https://www.culturebiosciences.com/resources/tag/white-paper) - [Technical Bulletins](https://www.culturebiosciences.com/resources/tag/technical-bulletin) - [Case Studies](https://www.culturebiosciences.com/resources/tag/case-study) - [Blog Posts](https://www.culturebiosciences.com/resources/tag/blog-post) - [Posters](https://www.culturebiosciences.com/resources/tag/poster) - [Announcements](https://www.culturebiosciences.com/resources/tag/announcement) - [Webinars](https://www.culturebiosciences.com/resources/tag/webinar) - [Videos](https://www.culturebiosciences.com/resources/tag/video) - [View All](https://www.culturebiosciences.com/resources) - [About](https://www.culturebiosciences.com/about) - [Login](https://console.culturebiosciences.com/login) [Contact](https://www.culturebiosciences.com/contact) [Login](https://console.culturebiosciences.com/login) [Contact](https://www.culturebiosciences.com/contact) Technical Bulletin November 10, 2026 # Continuous in-situ pCO₂ monitoring on the Stratyx™ 250 platform ![Continuous in-situ pCO₂ monitoring on the Stratyx™ 250 platform](https://www.culturebiosciences.com/hs-fs/hubfs/White%20Paper%20Feature%20%E2%80%93%20Gradient%20Background%20(15).png?width=860&height=745&name=White%20Paper%20Feature%20%E2%80%93%20Gradient%20Background%20(15).png) The analytical capabilities of Stratyx™ 250 continue to evolve, with the addition of viable cell density (VCD) monitoring followed by continuous in-situ pCO₂ measurement. When combined with Console’s ability to integrate and visualize these measurements alongside other process data, scientists can gain a more detailed understanding of changing process conditions and how they relate to overall bioprocess performance. In a preliminary 14-day CHO fed-batch study, continuous pCO₂ monitoring was evaluated using Hamilton CO2NTROL optical probes, with measurements compared against offline Nova Flex2 analysis. Recording data every five minutes generated 3,980 measurements per vessel compared with just 15 offline samples, a 265-fold increase in measurement density. The online measurements showed a strong correlation (r = 0.974) with offline results and revealed a prolonged low-pCO₂ excursion lasting up to 138 hours below 15 mmHg, a period captured by only two offline samples. These findings highlight how expanded monitoring capabilities, paired with connected data in Console, can provide deeper process insights and support more informed decisions throughout bioprocess development. 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