New data shared by one of our clients who scaled their process from our 250mL reactors up to 300L
Developing scalable fermentation processes, from strain screening to media optimization, requires running hundreds of bioreactor experiments at a small-scale that are accurate simulations of large-scale bioreactor conditions. Our high-throughput, parallel bench-top bioreactors have a 250mL maximum working volume, yet can be used to simulate production-scale conditions.
One of our clients worked with us to generate scale-up data for production of an extra-cellular small molecule in E. coli. After they developed their process in our 250mL reactors, they then moved to a 300L scale at a pilot plant. While the different geometries of the two reactors required different agitation and aeration rates, the similar dissolved oxygen (DO) percentage indicates that similar conditions were maintained in both reactors (as seen in Figures 1 and 2). As a result, the oxygen uptake rate (OUR) was very similar, demonstrating similar cellular metabolism between scales. Additionally, the final product titers were within 5% of one another.