Culture’s bioreactor capacity enables many different kinds of media studies. Media can be optimized using a DOE approach, which can identify the main media factors and interactions between components that can impact performance. Bioreactor capacity can be used to test feedstocks from different vendors or lots, or to shift from a defined research media to a complex medium for scale-up, or even to test the impact of different carbon sources and auxiliary feeds.
The data provided with each fermentation run allows deep insight into the metabolic state of the organism. In particular, off-gas data can provide information around the metabolism of an organism. Each of Culture’s bioreactors is fitted with continuous off-gas sensors that provide detailed online data that can be used to identify primary substrates being consumed and visualize the timing of metabolic shifts. This level of visibility allows rapid assessment of cellular metabolism for the purpose of media optimization, and accelerated media development timelines as a result. For more on media optimization in fermentation, including key challenges to keep in mind and best practices, check out this resource.